NAZORAT QILGAN HAYVONLARDA YUQORI NAFAS YULLARI DEVORI MORFOSTUZILISINING XUSUSIYATLARI.
Abstrakt
Nafas olish tizimining gistologiyasini o'rganish bir qator muhim ilmiy va klinik jihatlari tufayli tibbiy ta'lim va amaliyotning ajralmas qismidir. Tadqiqot maqsadi. Nazorat quyonlarida yuqori nafas yo'llari devori morfostrukturasining xususiyatlarini o'rganish. Tadqiqot materiallari va usullari. Bizning tadqiqot ob'ekti bir hil vivarium sharoitida yashovchi turli yoshdagi erkak quyonlar edi. O'rganilgan shaxslar klinik jihatdan sog'lom bo'lib, ularning o'rtacha vazni 2,3 dan 2,8 kg gacha. Tadqiqot uchun 18 ta erkak quyondan foydalandik. Burun devori, nazofarenks, halqum, traxeya va bronxlardan namunalar olindi. Olingan namunalar neytral formalinda mahkamlangan. Parchalarni mahkamlagandan va yuvgandan so'ng, ular 〖60〗^0 dan 〖100〗^0 gacha bo'lgan konsentratsiyali spirtlardan o'tkazildi va keyin ob'ekt kerosin bilan siqildi. Keyin biz mikroslaydlarni gematoksilin va eozin bilan bo'yash orqali qayta ishladik. Keyinchalik, mikrotom yordamida 7-8 mikron o'lchamdagi gistologik kesmalar olindi. Nafas olish yo'llari devorlarining histomorfologik tuzilmalarini o'rganish uchun biz MT 5300L markali yorug'lik mikroskopi ostida kuzatuvlar o'tkazdik. Natijalar har bir nafas a’zosining turli gistologik xususiyatlarini, masalan, nazofarenkdagi hujayra turlarining xilma-xilligini, halqumning uchta pardali murakkab tuzilishini, bir qavatli qatlamli qatlam bilan qoplangan traxeya shilliq qavatining o‘ziga xos xususiyatlarini ochib beradi. ustunli kipriksimon epiteliy. Rivojlangan aksirish refleksi kabi xarakterli xususiyatlar qayd etilgan bo'lib, bu hayvonlarning ushbu modelida yuqori nafas yo'llarining allergik reaktsiyalarini o'rganishga imkon beradi, ammo halqumda Klara va Langerhans hujayralarining yo'qligini ta'kidlash kerak. quyonlarda yo'tal refleksi. Tadqiqot quyonlarda bronxial daraxtning 32 ta tartibini, odamlarda esa 25 tani aniqladi. Xulosa. Shunday qilib, tadqiqotimiz natijalari quyonlarning nafas olish tizimining gistologik tuzilishini kompleks tashkil etishni tasdiqlaydi. Bu tizimning har bir komponenti, burun bo'shlig'i va nazofarenksdan bronxial daraxtgacha, o'ziga xos xususiyatlarga ega. Nazofarenks shilliq qavatida va burun bo'shlig'i devorlarida har xil turdagi hujayralar mavjudligini, shuningdek, halqum, traxeya va bronxlarning strukturaviy xususiyatlarini ta'kidlash muhimdir.
Mualliflar haqida
Adabiyotlar ro'yxati
Kargopoltseva DR, Katelnikova AE, Kryshen KL, Gushchin YA. Features of the respiratory system of animals used in preclinical studies that must be taken into account when modeling lung pathologies. Laboratory animals for scientific research. 2020;(4):71–85. DOI: https://doi.org/10.29296/2618723X-2020-04-08
Murgia N, Gambelunghe A. Occupational COPD—The most under‐recognized occupational lung disease? Respirology. Jun 2022;27(6):399–410. DOI: https://doi.org/10.1111/resp.14272
Brosnahan SB, Jonkman AH, Kugler MC, Munger JS, Kaufman DA. COVID-19 and Respiratory System Disorders: Current Knowledge, Future Clinical and Translational Research Questions. ATVB. Nov 2020;40(11):2586–97. DOI: https://doi.org/10.1161/ATVBAHA.120.314515
Zakaria DM, Zahran NM, Arafa SAA, Mehanna RA, Abdel-Moneim RA. Histological and Physiological Studies of the Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Bleomycin Induced Lung Fibrosis in Adult Albino Rats. Tissue Eng Regen Med. Feb 2021;18(1):127–41. DOI: https://doi.org/10.1007/s13770-020-00294-0
Cruz FF, Rocco PRM. The potential of mesenchymal stem cell therapy for chronic lung disease. Expert Review of Respiratory Medicine. 2 Jan 2020;14(1):31–9. DOI: https://doi.org/10.1080/17476348.2020.1679628
Blais-Lecours P, Laouafa S, Arias-Reyes C, Santos WL, Joseph V, Burgess JK, et al. Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis. Am J Respir Cell Mol Biol. Jan 2020;62(1):35–42. DOI: https://doi.org/10.1165/rcmb.2018-0397OC
Panova AE, Kulikova IB, Lagutkin DA, Vinokurov AS, Shulgina MV, Vasilyeva IA. Coronaviruses are causative agents of severe respiratory diseases. Tuberculosis and lung diseases. 2020;98(7):6–13. DOI: https://doi.org/10.21292/2075-1230-2020-98-7-6-13
Parekh KR, Nawroth J, Pai A, Busch SM, Senger CN, Ryan AL. Stem cells and lung regeneration. American Journal of Physiology-Cell Physiology. 1 Oct 2020;319(4):C675–93. DOI: https://doi.org/10.1152/ajpcell.00036.2020
Tolkach PG, Basharin VA, Chepur SV, Gorshkov AN, Sizova DT. Ultrastructural changes in the air-hematic barrier of rats during acute intoxication with fluoroplastic pyrolysis products. Bulletin of Experimental Biology and Medicine. 2020;169(2):235–41. DOI: https://doi.org/10.1007/s10517-020-04866-x
Ismoilov ZhM, Khamdamov BNU. Role of MUC5B in mucociliary airway clearance. Science and Education. 2023;4(5):344–52.
Qanday qilib iqtibos keltirish kerak
Это произведение доступно по лицензии Creative Commons «Attribution-NonCommercial-NoDerivatives» («Атрибуция — Некоммерческое использование — Без производных произведений») 4.0 Всемирная.
