ASSOCIATIONS OF FTO (RS9939609) AND PPARG 2 (RS18012820) GENE POLYMORPHISM IN CHILDREN WITH ABDOMINAL OBESITY AND METABOLIC SYNDROME
Abstract
The original article presents the results of a study of the distribution frequency of the FTO (rs9939609) and PPARG 2 (rs18012820) gene polymorphisms in children with abdominal obesity and manifestations of metabolic syndrome, as well as children with normal body weight. The results of the study are recommended for widespread use in the practice of family doctors, pediatricians and pediatric endocrinologists.
Objective of the study: to study the features of the association of the FTO (rs9939609) and PPARG 2 (RS18012820) gene polymorphism in children with abdominal obesity and manifestations of MS
Materials and methods. 72 children with abdominal obesity and manifestations of metabolic syndrome and 40 children with normal body weight were examined. A comprehensive anthropometric, anamnestic, clinical, biochemical and molecular genetic study was conducted to determine the distribution frequency of the FTO gene polymorphism (rs9939609) and PPARG 2 (rs18012820). Statistical processing of the obtained data was performed on a personal computer using the Statistica 10 program. In genetic studies, allele frequencies and allele combination frequencies were calculated and their compliance with the Hardy-Weinberg equilibrium using the c2 criterion with the calculated ones, rejecting the null hypothesis at P < 0.05.
Results. The results showed that the homozygous genotype of the FTO gene - T/T was found with a lower frequency in children with abdominal obesity and children with metabolic syndrome (c2=4,530, p=0,034), and the homozygous genotype A/A of the FTO gene was significantly more common in children with metabolic syndrome. In children with abdominal obesity, the Pro allele and Pro12Pro genotype were more common (85.5% and 73.6%) compared to children in the control group (76.2% and 60.0%). It was found that the Pro12Pro genotype of the PPARG-2 gene was statistically more common in children with MS - 84.6% compared to children who did not have this syndrome - 62.5% (c2=5,304, p=0,022).
Conclusion. The genotype A/A and allele A of the FTO gene (rs9939609) have a predisposing value to the abdominal type of obesity and metabolic syndrome. The predominant genotype in children with the abdominal type of obesity and manifestations of metabolic syndrome was Pro12 Pro, and in children with the abdominal type of obesity without metabolic disorders, the frequency of the minor allele 12Ala was statistically higher compared to children with manifestations of metabolic syndrome, which allows us to classify this allele as protective for the development of metabolic disorders
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List of references
Panasenko L.M., Nefedova Zh.V., Kartseva T.V., Cherepanova M.I. The role of obesity in the development of metabolic syndrome in children. Ros Vestn Perinatol i Pediatr 2020; 65(2): 125–132. DOI: 10.21508/1027–4065–2020–65–2–125–132 DOI: https://doi.org/10.21508/1027-4065-2020-65-2-125-132
Higgins V, Adeli K. Pediatric metabolic syndrome: pathophysiology and laboratory assessment. EJIFCC. 2017; 28(1): 25–42
Ubaydullaeva S.A., Mekhmonova S.U. The role of gene associations in the development of obesity in children. Medicine: Theory and Practice. 2019; 4(1):12-16
Heinzle S, Ball GD, Kuk JL. Variations in the prevalence and predictors of prevalent metabolically healthy obesity in adolescents. Pediatr. Obes. 2016;11(5): 425–433. DOI: https://doi.org/10.1111/ijpo.12083
Bairova TA, Sheneman EA, Rychkova LV, Ievleva KD. The FTO gene and its role in the development of obesity and overweight in children. Pediatrics. 2017; 96 (4): 186–193 DOI: https://doi.org/10.24110/0031-403X-2017-96-4-186-193
Zakharova IN, Malyavskaya SI, Tvorogova TM, Vasilyeva SV, Dmitrieva YuA, Pshenichnikova II. Metabolic syndrome in children and adolescents. Definition. Diagnostic criteria. Medical Council. 2016; 16:103-109. DOI: https://doi.org/10.21518/2079-701X-2016-16-103-109
Grechukhina E.I. FTO gene as a genetic risk factor for obesity development. Universum: Medicine and Pharmacology: Electronic sci. journal. 2019; 2(57): 123-128
Ievleva K.D., Bairova T.A., Sheneman E.A., Ayurova Zh.G., Balzhieva V.V., Novikova E.A., Bugun O.V., Rychkova L.V., Kolesnikova L.I. Protective effect of the G allele of the PPARG2 rs1801282 polymorphism against overweight and obesity in Mongoloid adolescents. Russ. J. Med. Biol. Iss. 2019; 7(4): 452–463. DOI: 10.17238/issn2542-1298.2019.7.4.452 DOI: https://doi.org/10.17238/issn2542-1298.2019.7.4.452
Foraita R. et al. Does the FTO gene interact with the socioeconomic status on the obesity development among young European children. Results from the IDEFICS study. Int. J. Obes. (Lond). 2015; 39 (1): 1–6. DOI: https://doi.org/10.1038/ijo.2014.156
World Health Organization. Obesity and Overweight. Factsheet Available from: http://www.who.int/mediacentre/factsheets/fs311/ru/ [Accessed 18 January 2022].
Fu M, Chen H, Li X et al. Association of Pro12Ala variant in peroxisome proliferator "activated receptor" gamma 2 gene with type 2 diabetes mellitus. Chinese J. Med. Gen. 2002; 19(3);234–238.
Biryukova E.V. Molecular-genetic, hormonal-metabolic and clinical aspects of metabolic syndrome: diss. … Doctor of Medicine. Moscow, 2009: 314
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